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The amplicon encoding the N-terminal section was digested with the restriction enzymes EcoRI and NcoI, the fragment corresponding to the C-terminal was digested with NcoI and XhoI.
2We are supposing that the terminal pipe section is made impervious.
Therefore starch observed in the terminal section could be transient starch instead of stored-starch.
The multiple alignment clearly showed the highly structured DBD domain of approximately 100 amino acids, located in the proteins amino-terminal section, which was the most conserved section of maize Hsfs.
Two of the basic side chains at the N-terminal end of the β-sheet section are also directed towards the NC helix non-bulged strand.
These results suggest a regulation flow from non-core TS section to non-core CM section through the core section and that the non-core CM section is the terminal part of the regulation flow.
Apoptotic cell death in deparaffinized tumor tissue sections was detected using terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) with the Klenow DNA fragmentation detection kit (Roche, Indianapolis, IN, USA).
The terminal section near the shoot apex was also high in starch.
Nevertheless, this is a minor detail, since the relative contribution of any seepage from the terminal section to the total discharge is O.
After the reaction of Terminal Deoxynucleotidyl Transferase Recombinant rTdT), sections were covered with plastic cover slips, incubating at 37°C for 60 minutes inside a humidified chamber; reactions were terminated by immersing the slides in sodium citrate (SSC).
Due to missing data on the 5'-end of the control region in about one quarter of the taxa, a terminal section of 34 bp has been excluded from the phylogenetic analysis, leading to an alignment of 1,908 bp for the combined dataset.
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