After PCR amplification, emulsions were broken using butanol, and the beads were washed, enriched, and terminal transferased before quantification and deposition onto a slide for sequencing.
After PCR amplification, emulsions were broken using butanol, the beads were washed, enriched, and terminal transferased before quantification and deposition onto a slide for sequencing according to manufacturer's instructions.
(B ) Quantification of Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining at 2, 4, and 6 dpa in WT and hs :ARF fins exposed to heat shock (left).
Quantification of terminal branch (TB) lumen length (see Materials and Methods) showed longer lumens in h674and homozygousgous embryos compared to wild-type embryos (Figure 11).
In contrast, measurement and quantification of terminal lung bronchiole diameters revealed a dose-dependent and statistically significant constriction of the distal airways, suggesting that normal Fltp levels are important for lung airway homeostasis.
Second, because of previous reports on UCP2 antibody problems (e.g. [ 40], we compared the antibody used for relative quantification (C-terminal peptide) with one raised against an N-terminal peptide (Fig 2 and 3).
The images were used to determine the total number of terminal bud branches and for quantification of total branch length as described [ 45].
Desbutyl-lumefantrine plasma concentration data below the limit of quantification in the terminal elimination phase did not result in any major model misspecification.
Elimination half-life for buprenorphine was 6.4 hours in the current study, which might even be underestimated as serum concentrations did not fall below the limit of quantification in the terminal phase.
The following three different methods were used to detect apoptosis: (1) labeling the free 3′-OH termini with terminal transferase (TUNEL assay); (2) quantification of the externalization of phosphatidylserine by annexin-V staining, followed by flow cytometry analysis; and (3) measuring cleaved caspases by immunoblotting.
Furthermore, colocalization with Synaptophysin demonstrated that 25% of axonal Eag1 was present in the membrane of presynaptic terminals (Fig. 1C and quantification in 4A).
