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Bronchoalveolar lavage fluid (BALF) was obtained during terminal procedure as previously described (7, 9).
For the terminal procedure, mice were anesthetized with Avertin and given a single, i.v. injection of DyLight® 488 labeled tomato lectin (100 µg) (Vector Laboratories)20.
The MPTP intoxication protocol, the chronic L-DOPA treatment, the clinical assessments, the terminal procedure and the characterization of the extent of nigrostriatal denervation were conducted as previously published [3], [11], [12], [13], [14].
Injections were made as a terminal procedure, after optical imaging and electrophysiological recording, and targeted to patches physiologically identified as object-selective.
In addition, blood sampling to measure metabolite or hormone levels is generally a terminal procedure so repeated sampling is not possible.
As a terminal procedure, the animal was deeply anesthetized and then injected i.v. with saline containing 10% sodium sulfide (200 mg/kg).
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Follow well documented Mac OS X Terminal procedures until you know what you are doing.
Be sure to only follow well documented terminal procedures while in terminal unless you know what you are doing.
Unfortunately the terminal sampling procedure precludes us from testing this directly but it is likely that dominants who fall in status will also exhibit a profound change in their transcript profile correlated with their lower status.
The evaluation of treadmill locomotion, over-ground locomotion and footprint analysis were compared at 6 weeks post-injury, just before the terminal electrophysiological procedure.
At the end of the conference, message is known at terminals The procedure explained above entails the following constraint on the common rate: (B.1).
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