Although we could not resolve the size or number of microscopic aggregates within each synaptic terminal by optical microscopy, under electron microscopy we did not observe any fibril within synaptic structures (Additional file 1: Figure S2).
For terminal meiotic phenotypes, microscopy was performed on live or ethanol fixed cells stained with DAPI (Sabatinos and Forsburg, 2010) using a 63× oil-immersion lens (PLApo, NA = 1.32) on a Leica DMR fluorescence microscope.
In a majority of patients infused with nanoparticles, the particles were confirmed to traverse into the terminal lobules by fluorescence microscopy.
DAB: 3,3'-diaminobenzidine tetrahydrochloride; dpc: days post-coitum; PBS: phosphate buffered saline; TBS: Tris-buffered saline; TEM: transmission electron microscopy; TUNEL: terminal deoxynucleotidyl transferase (TdT -mediated dUTdT -mediatedabelling.
The events occurring in the anther wall and microspores of male-fertile and male-sterile anthers were investigated by analyses of light microscopy, epifluorescence, terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling (TUNEL assay) and transmission electron microscopy coupled with electron spectroscopy.
We determined the relationship between the compression of collagen fibres and the deformation of axonal terminals using two-photon microscopy on stained serial sections.
Since the number of docked vesicles is a morphological correlate of neurotransmitter release probability at hippocampal synapses [16], we next analyzed by electron microscopy presynaptic terminals in stratum radiatum of the CA1 region (Figure 3A).
All animals underwent studies of intestinal functional capillary density (FCD) and leukocyte adherence on venular endothelium in the microcirculation of the terminal ileum by intravital fluorescence microscopy (IVM).
Direct parasitologic tests included the examination of 3 stool samples (both Kato-Katz and Ritchie techniques were used for each sample) and specific tests for Strongyloides stercoralis (Baermann test and agar culture) (1 ), optic microscopy of a terminal urine specimen, and Knotts test for microfilaremia.
In this study, three different techniques to confirm apoptosis were applied: routine microscopy, the TUNEL (Terminal-deoxynucleotidyltransferase-mediated d UTP-digoxigenin nick and labeling) method, and activated caspase 3 labeling.
Analysis of the purified HPyV12 VP1 lacking the 16-aa-long N-terminal using negative staining electron microscopy showed that this protein efficiently assembled into VLPs that were 45 50 nm in diameter (Fig. 2k).
