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He counted houses, high-rise apartment buildings, stadiums, schools, social clubs, offices, clinics, bus terminals, libraries , and a reservoir among his structures.
George Metesky, the one‐time "Mad Bomber," who for 16 years in the nineteen‐forties and fifties terrorized the city with the explosives he set off in theaters, terminals, libraries and offices, is going home to Waterbury today.
Since then - with varying levels of success - neglected or underutilized factories, warehouses, churches, schools, railroad terminals, libraries , movie theaters and even garages, have been recycled into retail and restaurant complexes, art museums, galleries and studios, lofts for living, office spaces, bookstores and condominiums.
For both the PB2 C-terminal truncation library screened against PB1- 676 757) and the PB2 N-terminal truncation library screened against importin α1, most constructs generated purified PB2-bait complexes strongly visible by streptavidin-western blot of the purified fractions (74 and 95% respectively; Fig. S3).
The N-terminal display system greatly reduces the selection of artifactual peptides, in comparison with a C-terminal fusion library displayed on lambda ([ 7] and our unpublished data).
Moreover, he said, grades 4, 5 and 6 are connected to the Internet; all classrooms have at least one computer, the technology lab has 34 Macintosh terminals , the library five computers and a multimedia center with computers is imminent.
This PB1 fragment used as bait was identified previously using ESPRIT from a PB1 N-terminal truncation library and, although purifiable at low yields, was shown to be unstructured by NMR (data not shown).
A comprehensive random PB2 C-terminal truncation library was constructed using an almost identical approach, but differing in cleavage of pYAN009 with NsiI and NotI located at the 3' end of the PB2 gene insert that had been cloned in frame with a 5' sequence encoding N-terminal TEV-cleavable hexahistidine tag.
From the PB2 N-terminal truncation library co-expressed with importin α1, fifteen clones exhibiting complexes were retested in 30 ml cultures in 100 ml shake flasks with purification on identical Ni2+NTA agarose resin in a gravity column format (Fig. S3).
In the settlement agreement, they also put terminals in libraries , but didn't ever get around to doing that.
The plasmid pYAN008 for N-terminal truncation libraries encodes an in-frame fusion of biotin acceptor peptide and an out-of-frame fusion of the hexahistidine tag sequence with the PB2 gene; while the plasmid pYAN009 for C-terminal truncation libraries encodes an out-of-frame fusion of biotin acceptor peptide and an in-frame fusion of the hexahistidine tag sequence with the PB2 gene (Fig. S1A).
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The phrase "terminal library " functions primarily as a compound noun, referring either to a software library used in terminal-based applications or a physical library equipped with computer terminals. The Ludwig AI indicates that the phrase is grammatically correct.
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