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Another set of 30600 trajectories of local docking but from a different starting point failed to produce a Ns-AtpD/Ns-AtpA-N terminal conformation with a better total energy of the complex.
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In the inactive variant, a third class of N-terminal conformation is observed, which arises from a conformational change in the position of F17.
Hence, STIM1 oligomerization is not sufficient for its conformational coupling with ORAI1 unless an extended C-terminal conformation is adopted.
An unusual C-terminal conformation has been detected in a synthetic decapeptide designed to analyze the stereochemistry of helix termination in polypeptides.
As the experiment is performed in the absence of YfiR, it suggests that an increase in the PG-binding affinity of YfiB is not a result of YfiB-YfiR interaction and is highly coupled to the activation of YfiB characterized by a stretched N-terminal conformation.
The R2-state of human hemoglobin features the same special C-terminal conformation of the α-subunit as observed in guinea pig hemoglobin [10].
The dimer, the building block of the hexamer of G40P, is formed following a cis-trans N-terminal conformation, where the two N-terminal tails come together (see Figure 4B).
We first analysed the N-terminal conformation change by a FACS-based assay to determine whether SB-218078 could block BAK N-terminal conformation change when cells were treated with the inhibitor followed by UV damage.
In a straightforward scenario, oligomerization of STIM1 likely helps to extend its C-terminal conformation controlled by domains within the first and third coiled-coil regions.
One construct with C residues at amino acids 18 and 63 (PAGE4 A18C) is sensitive to N-terminal conformation, and the other construct with C residues at amino acid position 63 and 102 (PAGE4 P102C) is sensitive to C-terminal conformation.
The mutation-induced extended STIM1 C-terminal conformation reflects an intramolecular transition, which likely exposes the CAD/SOAR domain and promotes interaction of full-length STIM1 with ORAI1, even in the absence of store depletion.
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