One major difficulty is that the state observation must only rely on the global system output i.e. the PDE state at the terminal boundary.
Notwithstanding certain well-known exceptions (e.g., restrained torsion of open thin-walled sections), such effects in isotropic cylinders are usually confined to a very small neighborhood of a terminal boundary or transition zone, and are typically neglected.
This region begins at the N terminal boundary of the Pfam GTP_EFTU domain and ends at the C terminal boundary of the predicted D2 domain, or since not all trGTPases have a predicted Pfam D2 domain even though they do in fact carry it, 100 amino acids after the C terminal boundary of the GTP_EFTU (the average length of D2 is 103 amino acids).
The inactive RFM4 and RFM10 mutants are located in the N- and C-terminal boundaries of a large predicted disordered region extending from amino acid ∼100 to amino acid ∼400 (Figure 7B).
Based on the online TMD prediction algorithm (http://dgpred.cbr.su.se/ ), no mutation was expected to change the N-terminal or C-terminal boundaries of the TMD.
The N-terminal and C-terminal boundaries of BdbZIP leucine zippers were demarcated following the criteria used for the bZIP proteins of Arabidopsis, rice and maize [ 10, 11, 15].
Interrogation of several N- and C-terminal boundaries (Supplementary Material, Fig. S1) in this expression system yielded a soluble and crystallisable polypeptide for hGBE1 from aa 38 700 (hGBE1trunc).
If the N- and C-terminal boundaries of a domain are defined as the first and last residue respectively of a series of residues under strong negative selection, the results will be reproducible and consistent among gene family or subfamily members.
They have the unique ability to self-catalyze their excision from the pro-protein(s) and in the process form a peptide bond between the flanking amino acid sequences that reside at the N-and C-terminal boundaries with the intein [ 1, 2].
Thus, the N-terminal boundary of the functional domain was placed at residue 850.
This region contains a palindrome which is immediately adjacent to the N-terminal boundary of the HC (Fig. 2A).
