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Court papers filed by Castle in the lawsuit say that it has an in-house laboratory that conducts daily testing to ensure its products meet the required standards, and that city inspectors are on site five days a week to test every truck leaving its terminal bound for city agencies.
These channels are voltage-independent but highly sensitive to [Ca2+]i due to the C- terminal bound calmodulin protein [16], [23] [28].
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Fig 5 d) and Fig S11 show that Beclin 1 N-terminal bound only weakly to Vps34, while this interaction is almost lost for Beclin 1 C-terminal.
Our previous research demonstrated that the complete DmsAL peptide (DmsAL1 43), which included the twin-arginine motif (15SRRGLVK21) and all three regions (N-terminal, hydrophobic, and C-terminal), bound to DmsD with a Kd of 0.2 μM when it was fused to the N-terminus of GST.
In particular, the study of proton-coupled events at the H-cluster and stabilization of a terminal hydride bound state have benefited from this approach.
The most recent structural evidence demonstrated that SYCP3 formed a tetramer, the N-terminal region bound DNA and the core domain assembled itself into the LEs (Syrjanen et al., 2014).
Of the six GCK-1 fragments tested, only the N-terminal fragment bound specifically to MPK-1a (Figure 6B).
The final model comprises full-length PPIL1 protein (except the very N-terminal Met) bound to CsA without any geometry violations and with good R-factors (Table 1).
The TR-FRET assay employs HiLyte Fluor 647-labeled apoA-I with N-terminal biotin bound to streptavidin-terbium.
However, despite being incorporated into either partial or complete endogenous NURD complex, both N-terminal fragments bound poorly the H3 tail peptide in in vitro pulldown assay.
The MBP-Vangl2 C-terminal domain bound GST-μ1 whereas mutation of the YYXXF motif blocked this interaction, consistent with a direct and signal-dependent interaction.
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