Based on the molecular weight, the cleavage seems to occur within or near the C-terminal BAG domain.
The C-terminal "BAG domain" of BAG-1 mediates the interaction with the Hsc70 and Hsp70 heat shock proteins [ 3], molecular chaperones that bind proteins in non-native states assisting them to reach a functional active conformation [ 4].
The human BAG family consists of six members (Bag1-6) which differ primarily by the presence of amino acid extensions at the amino terminus of a C-terminal BAG domain (Kabbage and Dickman 2008).
The C-terminal BAG domain is comprised of a bundle of three α-helices of which helices 2 and 3 mediate electrostatic interactions with the ATPase domain of the 70 kDa heat shock proteins, HSC70 and HSP70 (Briknarova et al, 2001; Sondermann et al, 2001).
At McCarran International Airport in Las Vegas, where airlines share terminals, bags are still randomly matched against claim tickets.
Given Kiffin's history, the bizarre manner in which he was fired, at an empty airport terminal with his bags headed to campus without him, was perhaps fitting.
So the airport decided on a new approach: instead of reducing wait times, it moved the arrival gates away from the main terminal and routed bags to the outermost carousel.
In response, the airport increased the length of the walk by moving the arrival gates away from the main terminal and routing bags to the outermost carousel.
On the other hand, the N-terminal sequences of BAG family proteins are different and have distinct functions [16], [32], [33].
All BAG-1 isoforms contain a C-terminal, evolutionary conserved BAG domain (Takayama and Reed, 2001) and a central ubiquitin-like domain (ULD), but the larger isoforms have unique N-terminal extensions.
Instead of heading out into the world and starting your vacation already, you have to stand around baggage claim watching other people's bags slowly snake around the terminal hoping that your bag didn't get lost somewhere along the way.
