Suggestions(1)
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Similarly in Figure 4B and C, the internal and terminal alignment gaps are nearly even-distributed.
The gaps refer to local alignment positions along r: in the output model sequence the gap length between two hits does not correspond to their distance along the chromosomal sequence, but rather to the distance between the hits' terminal alignment positions along r.
Similar(58)
Of the 168 aligned residues in the N-terminal alignment, only three are present universally in all sequences (Additional file 1).
The C-terminal alignment of the 65 kDa nACE with rat ACE revealed that the former was truncated at Ser482, and the sequence of the 90 kDa nACE ended at Pro629.
The C-terminal alignment was modified slightly such that the first position of the C-terminal Flag sequences aligned with the first position of the other sequences (Figure 4B).
(A ) C-terminal alignment of poxvirus A51R proteins with site of R321A substitution indicated by an asterisk.
Re-evaluation of the CDS by N-terminal alignment showed that selection of an alternative start codon in the same reading frame is in most cases sufficient to obtain an agreement.
After filtering for orthologs with congruent N-terminal alignments we determined 4,683 cliques and 6,667 gene pairs for the MPSS data, and for YALE-1 and YALE-2 4263 cliques with 4,395 pairs, and 2,185 cliques with 2,379 pairs.
Those marked with "‡" had signal peptides predicted only after manual inspection of the N-terminal alignments, and adjustment of the start sites to match those of the other two strains (see Methods).
These C-terminal alignments were also supported at the 2D level, as illustrated in Fig. 8 panel C, and include sequences which are predicted to form long helical structures.
Phylogenetic analyses were performed with N- and C-terminal alignments for protein and nucleotide sequences separately, and also in a combined analysis, concatenating the N- and C-terminal character matrices.
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