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All cell lines used were passaged in our lab for fewer than 4 months after resuscitation, and were used at the fifth through tenth passage in culture for this study.
The cell line was mycoplasma free and utilized before the tenth passage.
Microglia were collected from supernatants at 7 to 10 days after isolation while astrocytes were split and used from the fifth to the tenth passage [35].
The cells were expanded until the end of the tenth passage (corresponding to 26 cell doublings).
Parasites were not used after the tenth passage to carry out in vitro experiments.
For the subsequent experiments MSCs were expanded until the end of the tenth passage.
Similar(48)
A second passage worth consideration is the goal itself.
the second passage.
Third to fifth passage cells were used for next experiments.
First passage time statistics, such as the survival probabilities and first passage time densities are obtained analytically.
While the first passage seems to be Leibniz's position, he actually finesses this view in the second passage.
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