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Naturally, the above chain tends to integrate external resources in conjunction with their detailed requirements based on corporate external resource platforms.
However, due to its design, the available neomycin selection-based Cre expression plasmid occasionally tends to integrate into the genome and also yield only a moderate number of transfectants in liquid media.
While gamma-retrovirus demonstrates a bias towards integrating near transcription start sites and certain cell-cycle genes and oncogenes, the HIV-based Lentivirus tends to integrate preferentially within highly expressed genes [40] [44].
It is known that different retroviruses have different integration bias in mammalian genomes, e.g. Human Immunodeficiency Virus has preferences for transcribed units while Murine Leukemia Virus tends to integrate close to active promoters and CpG islands [ 1].
We made use of a combination of murine leukemia viral (MLV)- and lentiviral (LV)- based TrapSil vectors to obtain a greater diversity of targeted genes, since MLV tends to integrate close to active transcriptional start sites (TSS), while LV hits genes further downstream in their transcribed region [ 31, 32].
We have investigated the hypothesis that cytoplasmic organelle DNA tends to integrate preferentially into DNA in open chromatin regions by comparing nuclear organelle DNAs (norg) maps with chromatin status as revealed by DNase I hypersensitivity (DH) sites or Formaldehyde-Assisted Isolation of Regulatory Elements (FAIRE) (Boyle et al. 2008; Song et al. 2011).
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In "The Other America," Michael Harrington has described how social forces in the United States have tended to integrate the poor with the poor, and the old with the old.
But Australia also receives proportionally larger numbers from English-speaking countries such as the UK, Ireland and New Zealand, who tend to integrate more quickly.
About 500,000 are granted annually, on the assumption that married immigrants tend to be healthier and happier, and as studies show, tend to integrate more quickly into the American mainstream.
Third generation networks tend to integrate an ever increasing number of services.
In practical, these systems are not exclusive and often tend to integrate in one another because of the development cost.
Related(20)
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