Exact(3)
Crossing that temporary membrane, however, disrupts the conditions that made it possible in the first place.
This triggers a rapid build-up of osmotic pressure, leading to temporary membrane lysis and release of the polymersome payload within the cytosol.
In a GPR55-dependent, GDPβS and U73122-sensitive manner, LPI induced rapid and transient intracellular Ca2+ release that was associated with activation of charybdotoxin sensitive, large conductance, Ca2+-activated, K+ channels (BKCa) and temporary membrane hyperpolarization.
Similar(57)
Once expanded in vitro, juvenile chondrocytes are grown on a temporary polycarbonate membrane that is separated from the final tissue-engineered product at the time of packaging.
Permeability was appointed as key parameter for directly comparing temporary changes in membrane performance.
Defunctionalization is due to a number of effects that include temporary loss of membrane potential, inability to transport neurotrophic factors leading to altered phenotype, and reversible retraction of epidermal and dermal nerve fibre terminals.
Therefore we propose that the temporary increase in membrane permeability during cooling and freezing, as well as the freezing-induced increase in bleomycin concentration around cells will cause the bleomycin to enter cells during cryosurgery, when used at doses that otherwise would have no effect on the cells.
This apparent inconsistency could be explained if the mechanism of LDH release was temporary, for example via membrane blebs or transient pores present during TBHP exposure, followed by membrane re-sealing prior to the TER measurement.
Mg2+ and sucrose typically promote electro-transformation efficiency and cell viability because they stabilize the cell membrane from temporary distortion due to a high-voltage electric field, although they are not ascertainably advantageous for all bacteria (Wang and Griffiths 2009).
The effect may range from temporary permeabilization of cell membrane to irreversible electroporation resulting in a nonthermal ablation of tissue [ 21].
When microbubbles were exposed to ultrasound irradiation, it would produce a temporary increase of cell membrane permeability, thus promoting gene transfection into the target tissue.
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