In order to evaluate the temporal specificity between the lineage-specific genes and the evolutionarily conserved genes, the temporal specificity score, here defined as 1- H g)/log2(N), of the different sets of genes were determined, where H g) is the Shannon entropy that could be a good measure of uncertainty.
The large majority of the latter group of mRNAs are translationally repressed at mitotic entry and then translationally re-activated at mitotic exit, highlighting the precise temporal specificity of this gene-specific translational regulation.
We found that the entire two sets of lineage-specific genes showed an increased temporal specificity compared with the evolutionarily conserved genes (one-way ANOVA; p < 10-3).
In an "inverse mosaic" approach, germline mutants that develop tumors with high spatial and temporal specificity are studied by genetically manipulating specific regions of the tumor, or its environment, by expressing either the missing protein, or another protein, suspected to play a role in tumor development (Manfruelli et al., 1996; Qiu et al., 1998; Chiu et al., 2005).
SMART-cDNA probes were evaluated for their temporal specificity using quantitative real-time PCR (qPCR) to amplify the transcripts of stage-specific genes (data not shown).
Temporal specificity, in which TMS pulses are applied at different locations a few thousandths of a second apart, offers insight into the length of time that it takes for information to travel between different brain areas.
Nevertheless, developmental compensatory mechanisms and lack of spatial and temporal specificity limited the interpretations of these studies.
Here I describe strategies using the PhiC31 integrase to mediate recombination of transgenes containing attP and attB sites in cis to excise elements with spatial and temporal specificity.
The neoceptor neoligand pairing could offer spatial specificity by delivering the neoceptor to a target site, and temporal specificity by administering neoligand when needed.
In Caesarean-delivered control rats (Sprague Dawley), TH, D2R and D1R mRNA levels showed regional and temporal specificity in both absolute levels and developmental kinetics during the first week of life.
These regressive events depend on neural activity and occur in the early postnatal days known as the critical period, but what makes this temporal specificity is not well understood.
