Sentence examples for templates with total from inspiring English sources

However, it has been shown in Drosophila embryos that donor templates with total homology of 2 4 kb were the most efficient in editing induced by zinc finger nucleases (Beumer et al. 2013).

Variability in amplification may be introduced from several sources, namely the relative amount of chloroplast DNA to total DNA ratio and the tendency for inhibitory compounds to co-purify with DNA templates in total DNA isolation [ 37].

Panel a: Library constructed with total RNA as template.

The same site-specific primers were also used in PCR reactions with total genomic DNA template.

It is also important to notice that generated transgenic flax are stable in respect to expression of exogenously introduced genes since the field grown plants of third generation contained all transgenes and they are active which was confirmed by PCR analysis of transgenic plant with total mRNA as template.

with total genomic DNA as template [ 55].

To evaluate Math5 splicing in the cerebellum, we performed binary (2-primer) and competitive RT-PCR experiments with total adult cerebellar RNA as template (Figure S3).

With total genomic DNA of DSM1731 as template, primers clos-5 and clos-3 were used to amplify the homologous allele H1.

These optimized temperature profiles were then used for PCR amplification with total DNA of the cultivar Express as template.

Each RNA sample was reverse-transcribed (RT) using High Capacity cDNA Archive Kit to synthesize total RNA-cDNA templates with random hexamers.

A total of 62 PDB templates with different E-value cut offs for amino acid sequence similarity with BRP44 were obtained from PDB advanced search interface.