Sentence examples for templates with short from inspiring English sources
Recent studies have shown that linear templates with short (60 70 bases) homology arms, like single-stranded oligodeoxynucleotide (ssODN), could also be used in gene correction instead of plasmid-based construct.
Linear repair templates with short (∼30 60 bases) homology arms support the integration of base and gene-sized edits with high efficiency, bypassing the need for selection.
In this study, we demonstrate that linear templates with short homology arms (∼30 60 bp) support robust integration of both small and large (gene size) edits in C. elegans.
DOI: http://dx.doi.org/10.7554/eLife.06249.014 To further examine the relationship between the location of Gal4DBD and the ISW1a-remodeled nucleosome, we used two additional templates with shorter (24 bp and 50 bp) distances between the Gal4 binding site and the 601NPE.
Templates with a short region of homology limiting hybrid propagation exhibited a slow accumulation of transfer products, suggesting that for tested long homology templates hybrid propagation was not rate limiting.
The fabrication cycle for such NIL working templates with tunable feature sizes is maintained short and at a low cost.
After the exclusion of mGing-anchored fragments with short length or numerous HSPs, 21 unique flanking sequences were identified as templates for PCR primer designation.
To explore the consequence of APA, we quantify the abundance of RNASEH2C isoform with short 3′UTR in cells with in cis overexpression of KAT5, because it was possibly the major template for translation (Fig. 1A and 1D).
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