Sentence examples for templates with random from inspiring English sources

These short fragments were used as templates with random hexamer primer to synthesise first-strand cDNA.

These short fragments were used as templates, with random hexamer-primers used to synthesize the first-strand cDNA.

Each RNA sample was reverse-transcribed (RT) using High Capacity cDNA Archive Kit to synthesize total RNA-cDNA templates with random hexamers.

We primed cDNA synthesis on mRNA template with random oligonucleotides containing a constant 5' end.

cDNA was synthesized from the mRNA with SuperScript® II reverse transcriptase according to the manufacturer's protocol, 1000 ng of mRNA was used as template with random oligonucleotide primers.

RT-PCR was performed using the Gene Amp PCR kit (Roche Molecular Systems, Branchburg, NJ) as described in the manufacturer's protocol, using 1 μg of total RNA as template with random hexamers for RT priming.

As shown in Figure 1, the analysis system started from only one template initialized with random values, which is called Guard template.

According to the manufacturer's instructions, 0.5 μg of total RNA from each sample was used as a template with dN9 random primers (Ozyme, New England Biolabs), in a total volume of 100 μL.

One limitation of TdT, an enzyme widely used for end labeling of DNA oligomers, is that it is difficult to control the number of incorporated labels, because it shows template-independent extension with random nucleotides.

40 μl of RNA extract was used as template for RT with random primers.

For real-time RT-PCR, 2  μg aliquots of RNA as template were combined with random hexamers and reverse transcriptase, according to the manufacturer's protocol (Invitrogen).