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The effectiveness of the computer-based questionnaire is reflected in the results for this study with the students providing much more complete responses to the templates, with only 15%% of students missing a section from any of the templates, compared with more than half missing at least one section in the Template condition in the paper-based study.
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Amplification based on candidate EST primary templates was efficient, with only 13% of LAP pairs failing to generate amplicons.
Shown also are the warped original image to the template with SyN only (second row) and with SyN-CCFM (third row).
For the second step, the product of the first step was diluted 100-fold for use as the template, linear amplification with only the specific primer F536 was carried out, and the amplification product was diluted tenfold for use as the template for the following exponential amplification.
Negative controls for this experiment included both a "No RT" control (reactions with only template RNA and primers) and a "No cDNA" contamination control (reactions with primers and no RT product as template).
The resulting fragments were isolated from an agarose gel and used as templates in the second PCR with only the 5' BclI and the 3' AgeI primers giving rise to the final template.
A small aliquot (2-3 μl) of the first-round PCR product was used as template for generation of ssDNA with only the nested (N) primers.
We note that the homology exclusion cutoff used here is more stringent than that used by Bradley et al [ 13], who only excluded templates with a PSI-BLAST E-value < 0.05 but without sequence identity cutoff, and that used by Zhang et al [ 12], who only excluded the templates with sequence identity > 30% but without PSI-BLAST checking.
A head CT was performed in 38.8% (402/1036) of the visits that received a template with a head CT item, compared with only 11.1% (50/449) of those not receiving a template with a head CT item.
To build a new gene expression registration template, we used 249 embryos stained with only DNP ftz probes.
We only selected templates with at least 40% sequence identity (and e-value < 10−) with the pathogen query sequence.
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