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The fraction of horse reads recovered after treatment of TP1 and TP2 extracts with DNA phosphatase exhibited G→A cumulative misincorporation rates that were lower than those observed without DNA phosphatase treatment, suggesting that DNA phosphatase allowed preferential access to ancient DNA templates with lower levels of cytosine deamination.
When the ribozymes evolved to a more efficient form with a more complicated structure, they may no longer be able to act as good templates (with lower P LRTT, e.g., from 0.2 to 0.01, as shown in Figure 4B, bottom-left panel), then the chromosome would have an opportunity to emerge (provided that it adopted a strategy of circularity and self-cleavage).
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Increased amplification success with lower template amounts may also be seen.
34, 50 In homology modeling, the template structure of conserved amino acids in the two sequences will be enforced on the model of the target protein (see below) and therefore a receptor structure with high homology but in a different conformational state can be less suitable as a template than a structure with lower sequence homology.
The effect of childhood nutrition on menarche is contingent on the physiological template established in utero and the infant period, with lower birth weight and rapid growth in early childhood associated with earlier menarche [15], [20], [22], [23], [24], [25].
Consistent with this observation, preinitiation complexes formed on immobilized templates with transcription extracts lacking Ctk1 leave lower levels of the scaffold complex behind after escape.
DNA templates with extreme base-composition bias, primarily high G/C-content, are sequenced with lower efficiency on the GAIIx platform.
For the sample grown on the template annealed at 200°C, 0.7 orders of current magnitude increment was observed with lower Vt, 5.6 V.
The proportion of failed reads due to process-related problems are more abundant in traces with lower average read lengths while the opposite trend showing template-related failures increasing with increased average read length is true.
Pores were found to be larger for macroporous polymers produced from emulsions with lower internal phase volume ratios for the same energy input to the emulsion template.
The ultrahigh S concentration is obtained at an optimized condition, including a calcination temperature of 600 °C, whisker templates with a smaller diameter and a relatively lower carbon source to template ratio (1 5).
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