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The AAO templates with a nominal pore diameter of 80 nm were prepared by a two-step anodization process.
The protocol combines PCR-based synthesis and engineering of translation templates with a combined transcription translation system.
HCV RNA fragments were then generated by in vitro transcription of these DNA templates with a MEGAscript® T7 Transcription Kit (Invitrogen™, AM1334).
Di Giacomo et al. [6] prepared six surgical templates with a SLA method in four patients and compared the accuracy after implant placement.
For the sample grown on AAO templates with a 185-nm pore diameter, a combination of domain-wall motion and rotation modes was found.
After soaking of these foam hard templates with a phenolic resin, partially graphitized interconnected porous carbon monoliths can be obtained easily.
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Endogenous homologous sequences were also used as HDR templates, with an estimated editing efficiency of 25% (Fig. 2A).
The AAO templates with an average pore diameter of 25 nm were prepared by anodizing aluminum foil (purity 99.5%) at 20 V in 0.5 M sulfuric acid solution.
In summary, we have demonstrated the synthesis of carbon nanofibers containing linear mesocages arrays using AAO templates with an average channel diameter of 25 nm.
We prepared transcription templates with an N-terminal FLAG-tag sequence using the 'split-primer' PCR method (Additional file 2).
For the main part, he stuck to the de la Renta template, with a few subtle subversions.
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