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To rule out DNA contamination in the RNA preparations, the qRT-PCR controls were performed with RNA templates which did not show any amplification.
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Newer NGS platforms (3G) utilise single molecule templates, which do not require PCR amplification.
Many logo sites have templates which don't cost much.
A template which did not contain recognizable CTCF sites served as a negative control (lanes 11 12).
Compared to ProFunc's Reverse Templates method which does not depend on such knowledge, ETA is just as sensitive (61%) but significantly more accurate (100% vs. 67%).
Since 2D reads contain information from both template and complement strands, a non-redundant dataset would consist of 2D reads plus any remaining template or complement reads which did not get turned into 2D reads.
— which did not.
Which did he mean?
"Which did you prefer?
Which did I prefer?
Which did you portray?
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