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The quality and quantity of DNA templates were tested beforehand by means of a spectrophotometer (Nanodrop, Isogen).
The PSO templates were tested for face localization on the Yale B Face Database and compared to other methods based on anthropometric templates and Adaboost.
All templates were tested for both EFL and EF-1α, and none were found to encode both proteins.
To detect differences in the cDNA expression level for each sample set, a variable number of amplification cycles, between 20 and 24 depending on gene templates, were tested.
Different amount of genomic DNA templates were tested, and optimal annealing temperature in the first round (T a 1st) was also determined for the best resolution.
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This amount of competitor should subsequently be used in targeting experiments in which the ability of the LexA-Mad or LexA-TR DE) protein to recruit the Sin3/HDAC or N-CoR/SMRT compLexA-TR DEeir enzymatic activity to the immobilized temproteinare tosted (7).
Different concentrations 20-600 ng/ul) of genomic template were tested for a subset of samples, as well as different annealing temperatures 50-60°C 50-60°CeverandMgCl2 concentrationseveralMgCl2ut Pconcentrationsther almost invisibly faint bands that had a correct fragment size or mostly no bands at all for F. cinerea wsp.
At each step, every template is tested against the current sequence of content, in order to evaluate how much content can be consumed by this template; 3.
The input is the same as before: a content sequence and a set of templates; At each step, every template is tested against the current sequence of content, in order to evaluate how much content can be consumed by this template; The template that uses the most content with the least amount of error is selected and the sequence of content is reduced.
In order to establish the most appropriate conditions for the reaction the quantity of sample template was tested.
Each input and output DNA template was tested in 55 PCR reactions, each of which contained a tag-specific primer (not shown) and a common primer based on the plasmid backbone (Pr385).
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