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RNA templates were subsequently hydrolyzed in 0.5 M NaOH solution at 65°C for 20 min. The cDNA was further purified using a QIAquick PCR purification kit (Qiagen) and quantified using the OliGreen ssDNA Quantitation Kit (Molecular Probe, Hornby, ON).
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The templates are subsequently immobilized on streptavidin conjugated Dynabeads.
DSB-driven HR with dsDNA donor templates was subsequently used as well, e.g. in human cells [ 28, 68] and zebrafish zygotes [ 69].
Standardised amounts (200 ng) of template were subsequently reverse transcribed using the TaqMan Reverse Transcription Reagents kit (Applied Biosystems) in 20 µl reactions following the recommendations of the manufacturer.
In a subcutaneous rat model, this template was subsequently remodeled into mineralized bone tissue, including bone-marrow cavities.
The Al template is subsequently removed by acid etching, affording silicon nanoparticles wrapped by the highly conductive nitrogen-doped carbon.
RNA template was subsequently degraded in 20 mM EDTA and 50 mM NaOH.
The template was subsequently digested with DpnI.
Each DNA template was subsequently unzipped.
The atropine template was subsequently washed away from the MIP film with distilled water.
The mRNA synthesized from this template was subsequently amplified and mutated with Qβ replicase.
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