Sentence examples for templates were subjected from inspiring English sources

For PCR reactions, cDNA templates were subjected to PCR with Taq polymerase using gene specific primers (for pol λ: GCC CAG CTC AGC TCA GAG GAT GAA and CGT CGG TAA GAG CCA CAA GCC ACA; for actin: β-actin mRNA primers from Ambion).

SMRTbell templates were subjected to standard SMRT sequencing, as described [ 18, 19].

DNA templates were subjected to the sequencing reactions by using a BigDye Terminator version 3.1 Cycle Sequencing Kit (Life Technologies).

The DNA templates were subjected to single pass automated sequencing using the ABI3730 (Perkin Elmer, Foster City, CA, USA).

SMRTbell templates were subjected to standard SMRT sequencing using an engineered phi29 DNA polymerase on the PacBio RS system according to manufacturer's protocol.

Reverse transcription was performed with the Superscript RT kit (Promega Inc., Madison, WI) according to the manufacturer's instructions, and the resultant cDNA templates were subjected to PCR amplification.

Transcripts generated using closed-circular single-stranded M13 virus as the DNA template were subjected to RNA-seq.

In all the experiments, appropriate negative controls containing no template were subjected to the same procedure to eliminate or to detect whether any possible DNA contamination present.

Based on these quantifications, 300 molecules μL−1 per template were subjected to another round of 3D analysis, either separately or combined into a single reaction.

In addition, in all experiments, appropriate negative controls containing no template were subjected to the same procedure to exclude or detect any possible contamination.

Aliquots of 2 μL of cDNA template were subjected to Q-PCR using FAM-labelled scorpion primers (ATDBio, University of Southampton, UK).