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After washes and denaturation, templates were processed for immunoblotting with the indicated antibodies.
DNA templates were processed for the DNA sequencing reaction using the ABI-PRISM Big Dye Terminator version 3.1 (Applied Biosystems, Foster Cyty, CA) with both forward and reverse sequence-specific primer according to the manufacturer's guidelines.
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Unlike most recent studies, images were not registered to a template but were processed and segmented into gray and white matter in what is referred to as "native" space, although the images had been realigned into standard planes.
Images of micrographs from single sections were digitally recorded using a rectangular template, and recordings were processed and analysed using Northern Eclipse image analysis software (Empix Imaging, Inc).
Appropriate 'no template' qRT-PCR controls were processed similarly.
Planning CT tumor templates and MV-CBCT images were processed for the enhancement of tumors by using a Sobel filter or a blob structure enhancement (BSE) filter.
Forty-eight study samples, a calibrator sample and one no-template control sample (all in duplicate) were processed for each run.
A template must be processed together with a padding document given rise to a new document in some specification language, called target language.
While we did not encounter this during protocol development, it is possible that it may be observed as more samples – and particularly those with extremely low DNA template quantities – are processed with this assay.
A template-free control was processed for each experiment, establishing an absence of genomic contamination in the samples.
(If sequences of other taxa are processed, other templates will have to be selected).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com