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Computer-aided design and computer-aided manufacturing (CAD/CAM -fabricated surgiCAD/CAM -fabricatednterim prosurgicaltemplates were mand interimntal laboratory to facilitate comprosthesised surgery and templatesiate loading process.
Transcription templates were made by PCR and purified from agarose gel.
Four modified synthetic SMRTbell templates were made, each containing two 5mC, 5hmC, 5fC or 5caC modifications.
Synthetic SMRTbell templates were made as previously described by ligating several synthetic oligonucleotides [ 20].
Manual adjustments to the HHPRED-derived sequence alignments of ASP-2A with templates were made using JALVIEW [ 39].
Three 81-mer DNA templates were made to test the biocompatibility of the new linkage, each containing a single triazole.
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For template-guided assembly, existing surface atomic pattern or nano/micro features as templates are made use of.
Templates are made to match the patients' anatomy and include drilling guides and one or more osteotomy slits.
Another advantage of direct delivery is that the entire protocol requires no cloning: tracrRNA, crRNAs, and ssODN repair templates for short edits (<50 bases) are all synthesized chemically, and longer repair templates are made by PCR using oligonucleotides to code for the short (35-base) homology arms.
You will not be charged anything for the templates as all of the templates are made available completely free.
Constitutive negative/positive, point/sequential mutations involving residues Thr-210, and Lys-238 in WT TR2 (CMvector/Gas4) vectemplateemplate were made according to QuikChange XL site-directed mutagenesis kit (Stratagene) as described previously [PNAS].
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