Sentence examples for templates were loaded from inspiring English sources

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The templates were loaded onto flow cells.

Enriched templates were loaded onto an Ion 314 or Ion 316 Chip (Ion Torrent, Thermo Fisher Scientific).

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The index file is specified in the "templatefile" parameter of load_template_model, and the various templates are loaded into a Sherpa model instance assigned the ID specified in the "modelname" parameter.

Each well was filled with 250 mL of resin (3.5% Sephadex G-100, GE Healthcare) and the resin was packed by centrifugation at 3.000 × g for 5'; after addition of 150 mL of resin, the plate was re-centrifuged as above; 10 mL of the PCR template were loaded in each well and the purified reaction was collected by centrifugation as above.

Triplicate 5-μl real-time PCR mixtures, each containing Power SYBR green PCR master mix (Applied Biosystems), 312 nM each qPCR primer, and 0.5-1.5 0.5-1.5template were loaded onto a 384-well plate and run through 40 cycles on an ABS 7900HT sequence detection system (Applied Biosystems).

The ligated template was loaded into a 2% agarose size-select Invitrogen E-gel and selected at the 150 200 base pair range.

PCR reaction mix containing the diluted template was loaded onto Fluidigm's 12.765 Digital Array microfluidic chip.

The qPCR reaction mixture was made up of 12.5 μl of SYBR® Green PCR Master Mix (Applied Biosystems), 2 μl of a primer mixture containing 5 μM of each forward and reverse primer, and 10.5 μl of cDNA template was loaded into each well of the MicroAmp® Optical 96-Well Reaction Plate (Applied Biosystems).

The enriched template was loaded to a 314 or 316 (for mutIV-mix-2) chip and sequenced on the IonTorrent PGM using the Ion PGM 200 Sequencing Kit (wt-control; two runs) or Ion PGM 300 Sequencing Kit (C5K, A1, A6, H2, H12 & mutIV-mix-2).

Triplicates for each cDNA sample and buffer controls without template cDNA were loaded for each primer set.

Templated ISPs were loaded onto 316™ or 318™ Chips and sequenced according to Ion PGM™ 200 sequencing kit protocol.

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