Sentence examples for templates were initially from inspiring English sources

Throughout a complex polymerization of glucose at 180°C in the autoclave, scalable pencil-like SnO2 nanorod arrays employed as the templates were initially coated by organic carbonaceous layers on their outer surface.

Templates were initially denatured at 95 °C for 30 s, and targets were amplified over 35 45 PCR cycles at 55-63 °C (Table  1).> CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega) was used for MTS assay as previously described [ 11] to measure cell proliferation.

DNA templates were initially denatured at 95°C for three minutes, followed by 30 cycles with denaturation at 95°C for 30 seconds, annealing at 60°C for 30 seconds and, extension at 72°C for 45 seconds and finally, an extension at 72°C for five minutes.

The chloride anion template was initially removed by washing with copious amounts of 0.1 m NH4PF6/H2O.

To improve construct validity, the template was initially tested on two pairs of units by two pairs of reviewers.

Before the amplification, the template was initially denatured by incubation at 94°C for 9 min then the amplification was performed for 35 cycles of 94°C for 30 s, corresponding annealing temperature for 30 s, and 72°C for 1 to 2 min depending on the amplified product.

As described in the search strategy, protein sequences corresponding to predicted toxins, trafficking, presentation, processing and immunity domains were isolated using diagnostic domain architectures and gene-neighborhood templates, that were initially identified in previous studies [ 17, 18].

In the presence of nanoscopic templates, small copper nanoparticles were initially formed which on further oxidation gave copper oxide nanoparticles.

Plasmids carrying a 24-triplet AATTTA repeat with either the AAT or the ATT strand as lagging strand template for DNA replication were initially constructed as described in the materials and methods.

Candidates were initially validated using cDNA templates from all 16 genotypes included in the RNA-seq dataset.

Oligonucleotide primer pairs were initially tested using Chlorocebus aethiops DNA templates with a temperature gradient PCR (48 60°C) to determine the most appropriate annealing temperature for further analysis.