Sentence examples for templates were digested with from inspiring English sources
Templates were digested with DNase I and the cRNA purified by LiCl precipitation.
Original templates were digested with DpnI (Fermentas) and PCR product was transformed into E. coli DH5α cells (Invitrogen).
After 60 min of incubation at 42 °C, the cDNA templates were digested with DNase for 10 min at 37 °C.
Subsequently, 1 μg of amplified sample and 1 μg of amplified reference template were digested with DNaseI then labeled with Cy-5 dUTP and Cy-3 dUTP, respectively, using a BioPrime labeling kit (Invitrogen, Carlsbad, CA, USA).
The DNA template was digested with RNase-free Dnase I and RNA was precipitated with ethanol after phenol-chloroform extraction.
The DNA template was digested with DNase I, and the transcripts purified by illustra™ ProbeQuant™ G-50 Micro Columns (GE Healthcare, Buckinghamshire, UK) according to manufacturer's instructions.
The fragment, amplified by PCR using oligonucleotides oMMN01-135 and oMMN01-137 together with pMMN683 as template, was digested with NcoI and SmaI and then cloned into pTYB4 (New England Biolabs) digested with the same enzymes.
cDNA template was digested with RQ1 DNase.
The DNA template was digested with 1 U/μl RNase-free DNase I (Epicentre).
The methylated template was digested with DpnI (New England Biolabs) to leave the unmethylated mutagenesis product.
The original template was digested with DpnI, and the complementary strand was synthesized using the GFP_Nr primer.
