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Amplifications of the cDNA templates were detected by SYBR Green Supermix (Bio-Rad) using RT-PCR Detection System (Bio-Rad) and the cycle threshold values were determined by the MyIQ software (Bio-Rad).
Furthermore, we selected those proteins for which at least three weakly homologous and structurally related ligand-bound templates were detected by meta-threading using eThread [3].
Amplifications of the cDNA templates were detected by SYBR Green Supermix (Bio-Rad) using Real-Time PCR Detection System (Bio-Rad).
More pronounced topology-level improvements were achieved when new structural templates identified from the PDB were incorporated into the folding iterations; these templates were detected by the structure alignment program TM-align [ 6], which matches the TASSER models with each of the known proteins in the PDB to identify the templates that are structurally closest to the TASSER models.
Similar(56)
EPSCs that match the templates are detected with high sensitivity by convolving with templates; artifacts and noise transients are rejected (filtered out) because they do not match the template waveform and time course.
Instead, Ilex aquifolium templates are detected.
The incorporation of the fluorescent dye SYBR Green during PCR amplification of these templates was detected using Roche LightCycler technology and Quantitect SYBR Green chemistry (Qiagen).
However, no PCR product (using pooled cDNA from six ripening stages as templates) was detected for ACS degenerate primers but both sets of primers for ETRs produced a single band (Additional file 3: Figure S2) which after sequencing contained two different products.
Consequently, only polymorphisms occurring at high frequency in one PCR template were detected as double peaks in the chromatograms.
No DNA template was detected.
An equivalent ratio of wild-type to mutated template was detected using DNA and cDNA analysis.
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