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One methylated control sample, one unmethylated control and one 50/50 mixture of methylated and unmethylated controls were included in each PCR to ensure that methylated and unmethylated templates were both equally amplified.
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EpiTect® PCR Controls (Qiagen) was used to ensure that the reaction worked properly; one methylated control sample, one unmethylated control and a 50/50 mixture of methylated and unmethylated controls were included in each PCR to ensure that methylated and unmethylated template were both equally amplified.
All templates were tested for both EFL and EF-1α, and none were found to encode both proteins.
These templates were used for clinical documentation both for primary and specialised care in the region.
Application of this method permitted specific genotype identification for all cases with no cross-reactivity, even when both templates were added to the same tube.
For the bulk of the ASP-2A model structure both templates were simultaneously used, but they differed in the size of several loop regions.
Both residential templates were modeled with six different ventilation and filtration configurations (See Table 1).
Both RNA templates were shown to interact with the HCV-polymerase via initial Michaelis-complex formation and some subsequent intramolecular reactions.
All templates were sequenced in their entirety for both strands.
In both cases [ 158, 159], templates were extracted with ethanol.
dsDNA templates were prepared as described [30].
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