Sentence examples for templates were assessed from inspiring English sources

The integrity, purity and concentration of the extracted RNA templates were assessed by spectrophotometry at 260 nm and agarose gel (1.5%).

The DNA templates were assessed for HPV16 gene copy by real-time quantitative PCR using the ABI7700 sequence detector (Applied Biosystems, CA, USA).

The integrity of cDNA templates was assessed using gene-specific primers to β-actin.

The quality of the RNA template was assessed by the ratio of 28S:18S RNAs on a denaturing formaldehyde agarose gel after it was stained with ethidium bromide.

The average length of the amplified cDNA template was assessed in Agilent 2100 Bioanalyzer with the high sensitivity DNA chip kit (Agilent Technologies) to calculate the ratio of the desired miRNA ligation products (120 130 bp).

Following template reactions, templated libraries were assessed for quality using the Ion Sphere Quality Control Kit (Catalog # 4468656) and Qubit fluorometer (Life Technologies).

Fifteen formulations were generated using the Box-Behnken template, which were assessed for physicochemical and physicomechanical characterization.

The template items were assessed positively, it was understood that they were sufficiently broad, understandable and served as an excellent preliminary guide (FG 3).

The thermocycling conditions were 50°C for 2 min, 95°C for 10 min followed by 40 cycles of 95°C for 15 sec and 60°C for 1 min. Two non-template controls were assessed as negative controls for each experiment, and all experiments were carried out in double.

No-template controls were assessed in parallel to exclude contamination.

Run controls (positive, negative and no template control) were assessed to ensure that acceptable Ct values were met and that the reactions were performed correctly.