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Exact(10)
Application of this method permitted specific genotype identification for all cases with no cross-reactivity, even when both templates were added to the same tube.
Then, 2 µL cDNA templates were added to duplicate reactions performed in 96 well plates.
Primers and templates were added subsequently.
DNA was extracted, reaction vials were prepared for amplification, templates were added, and products underwent electrophoresis in separate rooms.
In order to reduce pipetting variability, we performed reaction batches containing primer pairs, and templates were added in the end.
Additionally, when stitching, the templates were added in equal molar ratios and PCR primers were added after the initial five cycles.
Similar(50)
It was found that the average TM-score [31] of the first identified templates can be increased by 2.5% with the introduction of the torsion angle restraints, where the difference between ANGLOR-predicted angles and the experimental angles in templates was added to the profile-profile alignment scores.
A standardized mixture of internal standard (SMIS) competitive templates is added to the reverse transcribed products prior to PCR.
Carbon nanoparticles, which act as hard template, were added under vigorous stirring.
Negative controls without template were added each time.
Two oligonucleotide primers (0.2 µM each) and <1 µg of DNA template were added to the reaction mixtures.
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