From the template, we obtained a striatal volume of approximately 22.3 mm3 by choosing a threshold of 50% of the maximum intensity of the striatum (Figure 3).
From the template, we obtained a striatal volume of approximately 22.3 mm3 with a threshold of 50% of the maximum intensity of the striatum.
By the penetration of mesoporous silica template with pure ANT, followed by the stabilization, carbonization and removal of the template, we obtained highly ordered mesoporous carbon rods (specific area 408 m2 g−1).
By using a specially designed holder for loading AAO membrane, which could control the distance from the evaporating source to the AAO template, we obtained a good conformal coverage.
With this template we obtained an amplification product with the D4Z4 but not with the DUX4c specific primers (Figure 1D).
However, when using cDNA template, we obtained the coding region of TFIIAγ5 and the first intron sequence using an internal primer for this species.
Since the SNPs were developed using the P. trichocarpa genome as a template, we obtained positional information for the Salix QTLs, projected them on the poplar genome, and identified the corresponding genomic intervals.
After sample electrophoresis of MSP reactions using bisulfited DNA as template, we obtained stronger PCR products using the unmethylated primer (UF3) for flutamide-treated mice DNA and faint bands correspondent to control samples).
Using human genomic DNA isolated from normal peripheral blood mononuclear cells (PBMCs) as the template, we obtained the MMP12 promoter −2000 −20000 to +97) fragment by PCR using the following primers: forward, 5'-TCCCC CGGGA CATAG AAAAA TTATC TAGTC CTACG TGTand' and reverse, 5'-CCGCT CGAGT GTAAA CTTCT AAACG GATCA ATTCA GTTT-3' (including SmaI and XhoI sites, respectively).
Using CRISPR/Cas9 system and a donor plasmid containing wild type coding sequence and homology arms (HA) as the repair template (Fig. S1A), we obtained the gene corrected iPSC clone (FUS +/+-HA iPSCs) from FUS +/G1566A iPSCs.
