Using genomic DNA from male or female Japanese flounder as templates, we found that the amplification products of Japanese flounder Ddx3 genes showed no sexual specificity.
With all kinds of templates, we found that recombinants were found more often among nontransgenic animals: more than 70% of all recombination events happened in animals that did not inherit the fluorescent array (Table 1).
With the practical use of the template, we found that sharing the latest knowledge about genome research and collaboration among various professionals were important in promoting a large-scale project that involves many research institutes.
Using human PP1C-γ as a template, we found that the conserved residues of Glc7 in the CD1-3 regions form a tertiary structure very similar to human PP1.
However, when we harvested RNA from stationary phase cells containing the P tar template, we found that the relative abundance of the ∼35-nt RNA was ∼50%, which was reduced to ∼10% when the transcribed-region −10-like element was mutated.
Using the immobilized template assay, we find that endogenous and exogenous H1 bind to the chromatin-assembled model promoter template, and that exogenous H1 potently represses transcription.
If amplification failure is a measure of template variation, we find a much greater variability among plasmid-associated virulence factors.
Measuring insertion of dCMP opposite template dG, we find that substituting Mn2+ increases the kcat ∼18-fold relative to Mg2+ (Table 4).
Based on experiments with nickel powder with and without the reticulated nickel template present, we found that both the particle size and sintering temperature influenced template morphology.
In addition to template switching, we found a high frequency of events in which one of the chromosomal loci became Ade+, reminiscent of earlier studies reporting "tripartite" recombination induced by a DSB on an ectopic template (Ray et al. 1989; Silberman and Kupiec 1994).
