Under single turnover conditions (e.g., 200 nM DinB and 100 nM DNA), DinB specifically incorporated dCTP opposite template-dG: we did not detect incorporation of dATP, dGTP, or dTTP (Table 3).
In order to clarify the meaning of the different domains and elements, we have made an effort to explicitly describe those element titles of the template that we did not deem self-explanatory.
Although other reviews are available in the literature [ 17, 18], none have been as exhaustive and none have systematically analyzed the existing models using a structured template, as we did.
Although the generated homology models do not incorporate the cofactor heme by nature of the program, the templates we chose do include the cofactors within the protein.
Dex and I had done a lot of acting before, but the rest were pretty much newcomers, so there was no template for how we did it – everyone just did their own thing.
However, if that were the case for the observed pauses, their occurrence would be correlated with the position on the template (which we do not observe) and their density would depend strongly on the applied force, as this force will destabilize the secondary structure ahead of the ribosome in the geometry used in these experiments.
While we did observe some Goldcoder templates in our own datasets, we did not find them occurring at the same frequency.
"We don't have a template of how we do it.
Daniel Benjamin, a former State Department counter-terrorism coordinator now at Dartmouth College, described Yemen as a "basic template for how we do counter-terrorism" with partnerships around the world.
However, it is possible that our cell-free system might not be completely appropriate or sufficient for cRNA synthesis from a vRNA template, and thus we do not exclude the possibility that IREF-2 also functions in unprimed cRNA synthesis.
