Using des-Arg bradykinin as a template, we designed a series of intramolecularly quenched fluorogenic peptide substrates for ACE2.
Using the DNA sequences of the Nipponbare reference genome as the template, we designed e-PCR primers by a Perl script based on the sliding window technique.
Using LC as template, we designed and synthesized a series of hydroxy-alkynoic acids and corresponding methyl esters, as putative, non-steroid BK channel activators.
With the assistance of soluble salt template, we designed and synthesized a Sn-core/CNT-shell nanocable anchored interconnected carbon networks structure via a simple CVD process.
Using the DNA sequences of the Nipponbare reference genome as the template, we designed 18,662,247 pairs of e-PCR primers based on the sliding window technique, which included 7,738,481 pairs in the intergenic region and 10,923,766 pairs in the genic region, accounting for 41.47 % and 58.53 % of the genic region, respectively.
Using the extended Chinook sequences as a template we designed primers (table 1) for genome walking.
Using the W325F mutant as a template, we designed two additional mutants, F146W and S229W, at the binding sites on the thumb and palm subdomains, respectively, and one mutant, Y271W, near the active site on the finger subdomain.
To test whether the observed ~500 bp fragment was spanning parts upstream of the sequence in the original template dsRNA, we designed a new probe for Northern blotting using the clone AmR9, located ~1.5 kb upstream of the original AP4a5 (see Methods).
In order to carry out this fidelity assay, we designed template h, which contained codons for each of the 20 amino acids and a C-terminal His-Tag.
Using the pyrrolidine-5,5-trans-lactam template, we have designed small, neutral, mechanism-based inhibitors of hepatitis C NS3/4A protease.
