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Using the most closely matched templates, we constructed LRRK1 models as described above for LRRK2.
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However, instead of creating an image as template, we construct three histograms as template, each corresponding to one color component.
Using threading analysis and multi-template modeling, we constructed a three-dimensional model of PDCD10.
Method: We constructed templates for various seizure types describing how often symptoms were selected by the participants.
(D ) Finally, we constructed template sequences that agreed with all the ordered pairs (see 'Materials and methods').
In order to answer the latter question, we constructed spatial templates from the activity of place cells in the first four laps of run (early templates) and the last four laps (late templates), and correlated them with the temporal sequences recorded during the previous sleep (early and late preplay).
In order to address this question, we constructed spatial templates from the activity of place cells in the first four laps of run (early templates) and the last four laps (late templates) and correlated them with the temporal sequences recorded during the previous sleep (i.e., early and late preplay).
We constructed the templates in two year increments for five different age groups: 7 8 years, 9 10 years, 11 12 years, 13 14 years, and 15 16 years.
To measure the actual translational efficiency of optimized and nonoptimized mRNA delivered directly to the cytoplasm, we constructed transcriptional template vectors encoding identical open reading frames as p96ZM651gag-opt or p96ZM651gag-NonOpt2 andve, and performed in vitro transcription to generate capped, polyadenylated synthetic mRNA.
We constructed mutation cassette template plasmids to facilitate subsequent production of the mutation cassette by PCR amplification (see Additional file 1: Figure S3).
We constructed new shape-average templates for the brain and VNC using nc82 confocal stacks, including an average male and an average female template brain.
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