Sentence examples for templates we added from inspiring English sources

To constrain transcription to a single round so that we can compare de novo PIC assembly on the two templates, we added 0.1% Sarkosyl immediately after the addition of nucleoside triphosphates (NTPs) for RNA synthesis following previously reported procedures using crude nuclear extracts (Hawley and Roeder, 1985; Kadonaga, 1990), which is also validated in our highly purified system.

For amplification of BAC-templates we added 2 μl of overnight culture to the PCR, while amplification of genomic DNA was carried out by adding 2 μl DNA with a concentration of 45 ng/μl to the PCR.

As template, we added 10 50 ng DNA to the reaction.

"We can do things around developer tools where we add JS templates, making your app use HTML5 app cache out of the box.

By sequencing PCR 'blank' negative controls, specifically PCR-amplified ultrapure water with no template DNA added, we were able to distinguish between taxa that had originated from the DNA extraction kits as opposed to DNA from other sources (such as PCR kit reagents, laboratory consumables or laboratory personnel).

Using the 'split-primer' PCR and the 37 cDNA clones as templates, we prepared the transcription templates by adding the sequences of the SP6 promoter, E01 enhancer region, and Biotin ligase site (Bls) to the 5'-end (Fig.  1a).

In order to reduce pipetting variability, we performed reaction batches containing primer pairs, and templates were added in the end.

Then, 2 µL cDNA templates were added to duplicate reactions performed in 96 well plates.

Primers and templates were added subsequently.

A standardized mixture of internal standard (SMIS) competitive templates is added to the reverse transcribed products prior to PCR.

DNA was extracted, reaction vials were prepared for amplification, templates were added, and products underwent electrophoresis in separate rooms.