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After the ligation reaction, a size range of cDNA templates was selected and amplified on a cluster station with the single-read cluster generation kit v2.
Size range of templates was selected by loading the entire processed sample on a 2% agarose gel and excising the gel region of 50-400 bp.
Excess adaptors were removed and a size range of templates was selected to go on the Cluster Station by loading the entire sample on a 2% agarose gel and excising the gel region of 50-400 bp.
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In template selection, the memory overhead was around 6.1 MB when 20% templates were selected to be the representatives.
Meanwhile, a part of LUT templates are selected to keep high quality of recovery images in the reconstruction process.
Templates are ranked by their SP-scores and the ligands corresponding to the top 100 templates are selected as template ligands for ligand virtual screening.
An appropriate template or set of templates are selected from a pre-defined list of templates that match the specified lipid abbreviations.
Thus at first ligand-bound structural templates are selected from the database of already known protein-ligand complexes by a threading (fold recognition) algorithm.
These templates were selected based on the best BLAST E-values found for the amino acid sequences matching with the query sequence, using a cutoff value of 0.5 for the alignment score.
Since the RNA templates were selected based on the poly-A tail, thus the antisense transcripts were likely polyadenylated.
Then the templates are selected to build the structure models for the query protein sequence using MODELLER [28].
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