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Twenty-five cycles of PCR were performed with the above cDNA product as templates, using the 5′ adaptor primer and 3′ gene-specific reverse primer.
The values present in this table are the asymptotical significance of the comparison between templates, using the Mann-Whitney test.
Adobe InDesign® was used to perform the rendering of documents, but it was also used for the authoring of templates using the format from Fig. 3.
The 3-D structures of AoXyn11A and its variants were modeled based on the three templates using the SALIGN program (http://salilab.org/DBAli/) and MODELLER 9.11 program (http://salilab.org/modeller/), and analyzed using the PyMol software (http://pymol.org/).org/
Reporter construct RNAs were transcribed from linearized DNA templates using the mMessage mMachine transcription kit (ABI).
Using these alignments, the sequences were threaded onto the templates using the program DEEP VIEW [97].
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Templates use the format shown in Fig. 3 and their graphical representation is illustrated in Fig. 4.
Previous synthesis processes employing colloidal hierarchical templates used the same material for the satellite and core particles.
For sample GM03756A, we amplified 1 ng of genomic DNA as template using the MyFi Taq (Bioline).
The standard 5′ end of the COI region was amplified for each individual DNA template using the primers LCO1490 and HCO219827.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com