Sentence examples for templates using standard from inspiring English sources

Before RT-qPCR was performed, all primer sets were tested for efficiency on DNA templates using standard PCR and each reaction resulted in a single prominent band on ethidium bromide-stained gels.

A cDNA fragment of KIAA1461 was PCR-amplified with exon specific primers KIAA1461for (5'-CTAGACCATGGGAAAAATGT-3') / KIAA1461rev (5'-ACTTGGAGACTGCTCCTCTA-3') and human genomic DNA as template using standard conditions.

This was determined from the vector template using standard dye-labelled primer protocols from Applied Biosystems and a model 373A automated DNA sequencing system (Applied Biosystems, Inc., CA, USA).

For KIAA1887 a cDNA fragment was PCR-amplified with exon 6 specific primers KIAA1887for (5'-CAGACCCCCTACTGTATTTC-3') / KIAA1887rev (5'-CAAAAGGTTAAAGCTTCCAT-3') and cDNA from a lymphoblastoid cell line as template using standard conditions.

SYBR Green PCR Master mix (Applied Biosystems, USA) was used for template amplification using standard protocol with specific primers for each of the transcripts examined [Additional file 2: Supplemental Table S1].

PDMS templates were fabricated using standard soft lithography approaches to contain channels 100 um wide and 20 um deep.

Rhesus genomic DNA obtained from the reference animal was used as the template for amplification using standard PCR procedures [ 20].

Chr = chromosome The specificity and linearity of the assays were measured using standard templates of known ratios of methylated:unmethylated DNA.

Standardized datasets are composed of: species occurrences' (using EML and Darwin Core, are published in IPT), biotic and abiotic analyses (using EML and spreadsheets filled using standard templates, are published in Metacat), functional and taxonomical analyses of metagenomes (using MIxS, are produced by MG-RAST).

Using standard template's layouts, we can create a precise preoperative flap design in the reconstruction of the tongue and of the floor of the mouth.

Normalization was performed to the EPI template available within SPM5 using standard preprocessing routines.