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This approach represents a "scaled-down" version used for sequencing complete nuclear genomes [ 16, 18, 20, 22, 48] and overcomes the significant limitations of sequencing AT-rich templates using conventional approaches [ 14].
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Table 1 Tabulation of the sample structures and the type of template used Conventional GaN Nano-ELO template Conventional InGaN/GaN QWs A B indium-rich nanostructures incorporated InGaN/GaN QWs C D LEDs with Stacked MQWs E F. The external quantum efficiency (ηext) of the LEDs is influenced by its internal quantum efficiency (ηint) and light extraction efficiency (ηextr).
The optimal annealing temperature for each primer pair was determined experimentally using conventional temperature gradient PCR (Taq DNA Polymerase, Life Technologies, Paisley, UK) from mouse heart cDNA as template.
Western analysis was performed using conventional techniques.
DNA was extracted using conventional methods [34] [42].
Those fleets used conventional Smart cars.
The camera uses conventional 35 mm film.
The first prototype MARVs used conventional wheels.
Otherwise use conventional super sized magnet.
The cDNA inserts were amplified from 2 µl of an overnight culture as template, using ExCyto PCR or conventional PCR with addition of commercially available, purified DNA polymerases (1.25 to 2.5 units) and their recommended buffers.
Create a template using brown paper.
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