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To speed-up, our method first aligns a target to all the templates using only the local alignment potential and then ranks all the templates using both the local and global alignment potentials described in Section 2. After ranking, only the first-ranked templates are used to build a 3D model by MODELLER for each target.
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All the experiments reported by Goldman et al. focus on promoter-proximal transcription events, in fact the entire length of the templates used both in vitro and in vivo is ∼116 bp (from the start site to terminator).
The brain-extracted MRI images in original space were then transformed to match this study-specific template using both linear 12-parameter affine, and 5th order non-linear transformation using Automated Image Registration (AIR; http://bishopw.loni.ucla.edu/air5/) [31].ucla.edu/air5/
The pre-processing pipeline includes: a) re-orientating images to the standard (MNI) template, b) bias field correction, c) registration to the MNI template using both linear (FLIRT) and non-linear (FNIRT) registration tools and d) brain extraction.
To achieve a precise normalization, we generated a study-specific SPECT template using both SPECT and MR scans of all patients and normal controls under study following a procedure described in detail elsewhere [ 21].
Recently, we report the growth of ZnO nanostructures on seed/catalyst-free substrates by introducing graphene as the template layer using both electrochemical and hydrothermal processes [14,23,26].
To alleviate calibration error and improve positional accuracy, single-molecule transcription traces that passed 85% of the template were aligned using both the stall site and the expected run-off length (Bintu et al., 2012).
Clean products were then used as templates for sequencing PCR using both forward and reverse primers and Big Dye terminator sequencing reaction, according to the manufacture's protocol.
The sensitivity of LAMP and PCR assays was done by using both template DNA and minimal CFU of bacteria.
One using bovine rhodopsin (1F88) as template and the other two using both bovine rhodopsin and potassium ion channel as template corresponding to two different regions of the query.
An optimized method of VBM was applied using both customized templates and prior probability maps, implemented using SPM5.
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CEO of Professional Science Editing for Scientists @ prosciediting.com