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The GaAs nanocrystal densities of the templates used for NW growth were 2 × 108 cm−2.
In expert-prepared templates used for recall and precision calculation, subjective external criteria may come into play.
This method is an intermediate step for creating the semiconductor nano-mound templates used for subsequent QD formation.
The TSHR TMD ECL1 is 14 residues long, whereas the lengths of the ECL1 of the templates used for modelling range from five to eight residues.
Furthermore, an inactive TSHR (TMD_In) structure was built based on the same three templates used for TMD_Ac, but in their inactive states (Table 1).
A sequence alignment of the ECL2 of the 16 GPCR templates, used for comparative modelling, with that of the TSHR TMD (Supplementary Fig. 1) identified bovine rhodopsin as having the highest amino acid sequence identity (32%).
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13561_2013_62_MOESM1_ESM.pdf Additional file 1: Structured template used for the review.
The DNA template used for RNA synthesis was y38f2ar.9 (YAC Y38F2AR: nt 56335 56636).
The template used for epPCR was the pET28a-celstrep construct harbouring the wild-type gene of CelStrep previously described (Amore et al. 2012).
The PDB template used for homology modeling was chosen using the profile build function in MODELLER.
The template used for each reaction was 0.1 ng semi-nested PCR product.
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