Sentence examples for templates to amplify from inspiring English sources

The gDNA and cDNA of TSS-AVRDC-2 and B-75 were treated as templates to amplify RubS and RubL sequences.

The plasmids were used as templates to amplify and subclone the IAPs into the pENTR/D-TOPO plasmid (Invitrogen).

Minigenes used as templates to amplify C, 5-mC or 5-hmC containing oligonucleotides (Figure 2 and Figure 5) were synthesized by Integrated DNA Technologies.

Non-detection of rare taxa in these analyses results from failure of DNA templates to amplify in PCR when present below a threshold concentration.

The KO-plasmids were used as templates to amplify the CA-hph cassettes with oligonucleotide pairs cynT1-5f/cynT1-3r cynT1-5f/cynT1-3r cynT1-5f/cynT1-3r3-3r (Table S2 and Figure S1).

The resulting cDNAs and total DNA were used as templates to amplify MpSIG1.

Genomic DNA from S. cerevisiae BY4741 was used as a template to amplify α-MF (Scα-MF) coding fragment.

The TE layer was used as a DNA template to amplify the rDNA sequence with NL1 (5'-gcatatcaataagcggaggaaaag-3') and NL4 (5'-ggtccgtgtttcaagacgg-3') as primers.

The genomic DNA was isolated from wild type A. carbonarius 5010 by using phenol-chloroform extraction (Andreou [2013]) and used as template to amplify 5' and 3' flanking regions of the gox gene with primers containing uracil overhang (Table 1).

Total DNA extracted from the fish gills and intestines were used as template to amplify bacterial 16S rDNA V3 region using GC clamp primer to identify specific bacteria of fish origin by PCR-DGGE technique.

Purified cDNA from a λ-phage expression library produced from worker fire ants was used as template to amplify a fragment of the GST transcript which was subsequently cloned and sequenced.