Exact(2)
Indeed, we have found that in some cases PfuX7 is the only DNA polymerase that produces a PCR product in combination with DNA isolated from e.g. plant material (Nour-Eldin, H. H., personal communication), human cDNA (Lange, J. B., personal communication) or when doing WHOPS on large plasmid DNA templates (this work).
Work in this area has focused on C-rich sequences such as the RNA oligonucleotide CCGCC [ 35] and DNA oligonucleotide (CCCG 3CC [ 36], which make particularly good templates (this work has used the activated RNA nucleotide analogues 5'-phosphoro 2-methyl -imidazolidess, which - unlike standard RNA nucleotides - undergo efficient and regiospecific template-directed incorporation into a copy strand).
Similar(58)
The CNCs used as the starting template in this work have uniform fiber diameter, coil diameter, and coil pitch, and the average diameter of the fiber is about 80 nm (Additional file 1: Figure S1).
The Bloomberg Administration's HomeBase program provided a template for this work.
The templates used in this work are cheap, commercially available and this template carbonization method, especially without any activation process, can be readily extended to prepare other kinds of nanoporous carbon.
Due to the good quality of the polymeric templates used in this work, the diameter distribution within a given sample remains very narrow.
This shell is similar in concept to the static-region "template" described within this work.
Considering that these samples are potentiostatically electrodeposited and the AAO template used in this work is extremely disordered, this result is remarkable.
The surface morphology of a typical rib-patterned Si (1 1 10) template used in this work is shown in Fig. 1b with a three dimensional (3D) rendering of the AFM image for an area of 2 × 2 μm2 and with the ribs' orientation aligned along [1 −1 0] as a lower right inset in Fig. 1a.
Similar to previous studies [23], [24], the DNA template designed for this work was a fragment of 353 bp DNA containing the 147 bp nucleosome positioning 601 sequence [25], flanked by two arms of different lengths (79 bp and 127 bp).
Several different silicon templates were used in this work.
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