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as templates, the structures of either SiCYS-N or SiCYS-C showed similarity as those using Orzyacystatin I as templates (data not shown).
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The structure of the binding domain (L36 scFv) was modeled by comparative modeling [23] using as template the structure of 2GHW.B from the Protein Data Bank (PDB) [24].
Because the RNA polymerase subunits RPA2, RPB2 and RPC2 are similar in structure, we used as our common template the structure of RPB2 from S. cerevisiae [ 42, 43].
The pdb files for human eEF1A1 and eEF1A2 were generated at Swiss-Model Server using as template the structure of yeast eEF1A (PDB ID: 1F60, chain A).
Finally, segmentation was completed by fusing the optimal 3D template and the "structure of interest".
Second, the study provides a template of the structure, content and process of service delivery that can be replicated.
The model is first built by transferring the Cα coordinates of the template structures to the aligned residues in the query sequence.
This is done by directly transferring the Cα coordinates of the template structures to the aligned residues in the query sequence.
Depending on the template structures, the carbon patterns can be quadrangular or hexagonal in shape.
Briefly, the target sequences were aligned with the template structures, and coordinates from the templates were transferred to the targets TRR1 and KRE2.
The fragments in the aligned regions are used directly from the template structures and the unaligned regions are modelled with ab initio simulations.
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