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When the sequence of the TSHR TMD showed a deletion compared to the sequences of the templates, the additional residues were deleted from the templates plus one or two residues of the templates at each side of the deletion.
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In Sections 4 and 5 we define two additional problem templates: the Outside Vector Multiplication Template, and the Multiple String Vector Multiplication Template, and describe modifications to the algorithm of Valiant which allow to solve problems that sustain these templates.
If cutting out additional pieces, such as paw pads, lettering, eyes, etc., pin the additional templates to different pieces of felt and cut these out as well.
Most of the CNT literature claims that the additional template synthesis used in the CVD technique is a promising way to synthesise well-aligned CNT structures with control over diameter, size uniformity and density [6, 7, 8, 9].
Digestion of the template eliminated amplification of the additional target and allowed the identification of 4 more mutant alleles (Additional File 1).
Primers were designed with the Primer3Plus web interface [ 88] using the sequencing option and the PN40024 genome sequence as the template (Additional file 14).
Nevertheless, weak polyelectrolyte LbL templates (such as PAH and PAA) offer the additional advantage of an adjustable pH-dependent degree of ionization, which is a key parameter when in situ synthesis process (ISS) approach is used.
With different updating methods for the weights, two detectors are proposed: Hard Decision for MSTR (HD-MSTR) detector and Soft Decision for MSTR (SD-MSTR) detector, where HD-MSTR obtains the template based only on the previous detected symbols, while SD-MSTR constructs the template with additional information from the decision variables.
In Study 2, the procedural information for the experiments is much more similar between the Titles Template condition and the No Template condition in Study 2. Similar numbers of observations are recorded in the 'Step-by-step' section of the template, with additional observations recorded in the Discussion and Conclusion sections, as shown in Fig. 11.
In some rare cases when helices V and VI were shorter than in the template, additional residues from ICL3 were added to keep the helical structure in helices V and VI the same as in the 5HT2B construct.
This second step is crucial to retrieve Illumina reads that correspond to low-quality regions of the template (Additional 1: Figure S1).
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