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The PCR yield is the product of three efficiencies: (i) the annealing efficiency is the fraction of templates that form binary complexes with primers during annealing, (ii) the polymerase binding efficiency is the fraction of binary complexes that bind to polymerase to form ternary complexes and (iii) the elongation efficiency is the fraction of ternary complexes that extend fully.
For this reason, small diameter Mo NWs cannot be synthesized from the templates that form large bundles such as transported Mo6S2I8 NWs.
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The three complementary oligonucleotides (one primer and two templates) that hybridize to form a duplex with a protruding 5' end were used to create two substrates for DNA-polymerase reactions.
The cleavage mechanism typically employs two PNAs to mediate the induction of sequence-specific stretches of ssDNA in a dsDNA template that can form a substrate for a ssDNA-specific endonuclease.
DOI: http://dx.doi.org/10.7554/eLife.04970.006 To investigate how H-NS filaments affect transcript elongation by RNAP, we engineered a transcription template that could form λPR promoter-initiated, halted A26 elongation complexes (ECs) on a 26 nucleotide C-less cassette placed upstream from the bgl DRE and URE in the antisense direction.
Several research groups, including that of Martin Rinard, an MIT professor of electrical engineering and computer science, have developed templates that indicate the general forms that patches tend to take.
At the interfacial area of these templates, PBCA nanoparticles create deposits that form the particle wall, as the result of a partial filming process.
Testing for statistical evidence that expressions were paraphrases, the system compiled templates or patterns that formed the backbones of equivalent sentences.
Cut the meat pieces into shapes that form the dress pieces as per the templates.
The X-ray crystallographic data of compound 12 bound to the active site of squalene synthase provided an important insight into the binding mode of this alternative template that formed 11-membered ring conformations with an intramolecular hydrogen bond.
These regions govern interactions with the DNA template and the RNA/DNA hybrid that forms between the template and nascent transcript [25].
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